CTNNB1 and malignant colon neoplasm: In contrast, it increased the expression levels of intestinal epithelial differentiation markers such as KRT20 and MUCs. These changes in gene expression essentially recapitulated those observed upon the knockdown of CTNNB1 (Fig. 2B and Supplementary Fig. 2A, B), suggesting that PAF1C plays a key role in controlling the expression of genes that are essential for colon cancer stemness and is induced by stable β-catenin.