Thirdly, we noticed that, P. distasonis, considered a protective species attenuating colon tumor formation via blocking toll-like receptor 4 signaling pathway and Akt activation in HFD-induced CRC mice, and exhibiting decreased abundance in the CRC mice12, was specifically elevated in EBW-CRC but not in lean-CRC, indicating a possible difference between the gut microbiota of mice and human. This evidence concerns the gene TLR4 and colonic neoplasm.