Thus, we sought to develop cell-type-specific random forest (RF)-based classifiers for total Mono (VCAN+ Mono, Classical Mono, IFN- Non-classical Mono and IFN+ Non-classical Mono), CD4+ Tn 2, CD8+ Tn 2 and Treg to diagnose preeclampsia based on our scRNA-seq data and the results of the expression analysis above (Fig. 6a). This evidence concerns the gene VCAN and preeclampsia.