Laser Doppler showed a significant decrease in perfusion recovery in C57BL/6 J mice ischemic muscle treated with RCAN3-expressing plasmid vs. control plasmid (day 14: control plasmid 61.9 ± 1.74 vs. RCAN3 plasmid 49.8 ± 3.4), indicating that increased RCAN3 levels impair perfusion recovery in PAD (Fig. 5c). Here, RCAN3 is linked to peripheral arterial disease.