Based on the bioinformatics analysis or miR-17 and miR-20a predicted targets using the mirdb target prediction database46,47 and Targetscan7.248 that identified RCAN3 as a potential common target of miR-17 and miR-20a, we first examined whether VEGF165b inhibition modulates RCAN3 expression in experimental PAD models. Here, RCAN3 is linked to peripheral arterial disease.