Comparing doxycycline treated (DOX + ) to untreated cells (DOX-), we observed significant increases in several signaling pathways known to impact cancer cell proliferation and survival, including increased phosphorylation of AKT1/2/3 at T308, STAT3 S727, and p70 S6 kinase, to name a few (Fig. 6b). This evidence concerns the gene AKT1 and cancer.