To investigate the role of the ARQ62L and ARE81Q mutations in hepatoma cells, we constructed stable expression of ARQ62L and ARE81Q respectively in HCC-LM3 and MHCC-97H cells and certified the overexpression level of AR mutations respectively on mRNA and protein levels (Fig. 2a, b and Supplementary Fig. 2a, b). The gene discussed is AR; the disease is hepatocellular carcinoma.