Hence, to assess whether integrated microbiota compositional and functional analysis could predict T1D diabetes development, we used receiver operating characteristic (ROC) curves to predict outcomes based on microbiota compositional assessment after 0, 4, and 6 weeks of treatment, as well as based on microbiota functional features (including fecal levels of flagellin, LPS, TLR2 ligands at weeks 8 and 10 of age, microbiota-IEC distance, week 10 fecal IgA coated bacteria, and flagellin/LPS specific antibodies circulating levels at week 8 of age and euthanasia). Here, TLR2 is linked to diabetes mellitus.