Anti-Flag Co-IP assays showed that removal of 1–94 amino acids region of CLIC3 or 501–758 amino acids region of NAT10 abolished the interaction between CLIC3 and NAT10 (Fig. 6I, J), indicating that CLIC3 and NAT10 formed a protein-protein complex through the interaction between Gluta- thione S-transferase (GST) N-terminal domain of CLIC3 and N-acetyltransferase domain of NAT10 in bladder cancer cells. This evidence concerns the gene HPGDS and urinary bladder cancer.