Collectively, our results suggest that Ly6Ehi neutrophils not only serve as a predictive biomarker for immunotherapy response in mice bearing 4T1 tumors but also: (1) are functionally involved in the mechanism of response; (2) operate upstream of T cells; (3) can be induced by an entity other than the adaptive immune system or host (e.g., tumor-intrinsic STING signaling, via IFNα or via IFNγ through yet-to-be characterized mechanisms); and (4) contribute to anti-tumor immunity by directly activating cytotoxic CD8+ T cells via IL-12b (Figure 5G). This evidence concerns the gene CD8A and neoplasm.