Using this model, and in-conjunction with mass cytometry (CyTOF) and flow cytometry, we confirmed that mutagenesis resulted in tumors with a higher degree of immunogenicity, characterized by reduced numbers of immunosuppressive cells (e.g., G- and M-MDSCs and PDL1+ cells), increased numbers of anti-tumor immune cells (e.g., activated B and T cells) and elevated Granzyme B levels (Figures 2B–2D and S1B–S1I). This evidence concerns the gene GZMB and neoplasm.