PANX1 and osteosarcoma: In addition, to gain a deeper understanding of Purinergic gene expression in a variety of tumor cells, and based on the immunofluorescence images obtained from the HPA website (Figure 1F), we found that the Purinergic gene PANX1 was significantly expressed in the cell cytoplasm in both A431 cell line (Human epidermoid carcinoma cells) and U-2-OS cell line (Human osteosarcoma cells).