Therefore, to investigate whether fluvoxamine plays a role in C9orf72-ALS and affects the nuclear pore complex (NPC), we pretreated NSC34 cells with 10 μg/mL fluvoxamine and overexpressed (G4C2)31 RNA in NSC34 cells, followed by immunostaining and RT-qPCR. The gene discussed is C9orf72; the disease is amyotrophic lateral sclerosis.