To compare cortical migration across our five models of dystroglycanopathy, we performed immunostaining for the upper layer marker Cux1 (layers II/III-IV) and the deep layer marker Tbr1 (layers III, VI) in P30 somatosensory cortex (Figure 2A–B). Here, CUX1 is linked to neuromuscular disease caused by qualitative or quantitative defects of alpha-dystroglycan.