An unexpected finding was that DHF treatment in \documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{upgreek}\setlength{\oddsidemargin}{-69pt}\begin{document}$$\text{ER}\alpha$$\end{document}ERα−/− mice, regardless of sex, caused an increase in anxiety, as assessed with distance traveled and speed, but paradoxically a decrease in anxiety, as assessed by freezing episodes. Here, ESR1 is linked to Anxiety.