To validate the bioinformatics analysis results, IHC staining was applied to investigate the expression of immune microenvironment markers (dendritic cell CD CD1α, CD4 T cell, CD4) and ferroptosis markers (CHMP5 and DDIT3) in normal ovarian tissues and low- or high-grade ovarian cancer tissues (Fig. 7). The gene discussed is DDIT3; the disease is ovarian cancer.