TMEM25 and adenocarcinoma: Indeed, depletion of TMEM25 induces an abnormal cyst formation in 3D culture of MDCK cells, indicative of an impaired cell polarity (Fig. 4C); and TJ structure alteration and subsequent impaired polarization are considered to result in a multitude of diseases, especially adenocarcinoma of various organs (Royer and Lu, 2011; Muthuswamy and Xue, 2012; Garcia et al, 2018; Zeisel et al, 2019).