To evaluate the role of NME4 in NAFLD progression and lipid accumulation, we first generated an Nme4 knocked down mice by injecting AAV type 8 virus expressing Nme4 shRNA (AAV-shNme4) via tail vein, to primarily knock down Nme4 in mice liver, and fed mice with HFD for 12 weeks (Fig. 3A). The gene discussed is NME4; the disease is metabolic dysfunction-associated steatotic liver disease.