At each tertile of the exhaustion-state trajectory (time 1, 0–33% of the pseudotime; time 2, 33–66% of the pseudotime; time 3, 66–100% of the pseudotime), we divided TCGA melanoma patients into low and high groups (median cut-off) based on the average abundance of CD8+ T cells. The gene discussed is CD8A; the disease is melanoma.