PTPRC and non-small cell lung carcinoma: In parallel, given the low sensitivity of CellSearch in NSCLC [17, 18, 30], we used hematopoietic blood-cell depletion combined to immunofluorescence staining and fluorescence-activated cell sorting (FACS) to isolate single Hoechst+/CD45−/Cytokeratins+ (called CD45−/CK+) cells from 30 mL blood samples, according to a previously reported experimental workflow [26, 27].