To further elucidate the mechanism via which GSDMD in macrophages promoted orchitis, we performed RNA-sequencing (RNA-seq) of macrophages (CD45+CD11b+Ly6G−F4/80+) sorted from the testes of WT and Gsdmd−/− mice treated with UPEC (Fig. EV4A). This evidence concerns the gene PTPRC and orchitis.