GSDMD and orchitis: Consistently, the results of CASA showed that sperm count, frequency of motile sperms, ratio of progressive sperms, and VAP, VSL, and VCL of motile sperms were significantly higher in Gsdmd−/− mice than those in WT mice (Fig. 2C,D), indicating that GSDMD deficiency significantly enhanced the quality of sperms during orchitis.