LINC02454 and central nervous system cancer: To assess whether LINC02454 activity regulates glioma cell sensitivity to TMZ, we transfected U251 cells with locked nucleic acids (LNAs) to knock-down (KD) LINC02454. qRT-PCR analysis of control and KD cells confirmed LINC02454 downregulation to approximately a third of levels seen in control cells (Fig. 3A).