To explore the potential pathophysiological role of CUL5 in AMs during asthma exacerbations, we generated myeloid-specific Cul5-deficient mice (LysMCreCul5fl/fl) by breeding Cul5-floxed mice (Cul5fl/fl) with Lysozyme M-Cre mice (LysMCre) (Supplementary Fig. 2). This evidence concerns the gene CUL5 and asthma.