To assess mRNA and protein expression of the two POM121(A/C) genes, a set of human CRC cell lines (CIN + MSS + : BRAFV600E HT29, KRASG12V SW480; CIN- MSI + : KRASG13D HCT116 [53]) and HEK293T cells (non-cancer KRAS wt control) were subjected to RT-qPCR (Fig. 1C) and Western blot (Fig. 1D) using primers and Abs directed against the N- or the C-terminus of full-length (FL) POM121A/C. The gene discussed is POM121; the disease is cancer.