A dramatic reduction of CHAT+ motor neurons was observed in the ventral horns of the lumbar spinal cord of SOD1G93A mice when compared to their wildtype counterparts (Fig. 7D, E; group effect: F(2, 11) = 47.61, ****p < 0.0001, one-way ANOVA), consistent with known pathological features of this ALS mouse model. Here, CHAT is linked to amyotrophic lateral sclerosis.