The main aim of this study was to quantify the selected SASP factors (IL6, IL1b, CXCL1, TNF-α) and senescence markers (p16, LMNB1) transcript expression and correlate this data with patients’ clinical features to clarify their physiological and pathological roles in HNSCC. The gene discussed is CXCL1; the disease is head and neck squamous cell carcinoma.