We further analyzed gene-gene interactions of deregulated glucose related genes in breast cancer, and it was found that deregulated glucose related genes show high interactions with PFKFB2, PFKFB4, FBP2, PCK2, ADH5 and other genes involved in glucose metabolism (Fig. 8A). The PPI grid was developed by employing STRING and the confidence score value of the network was > 0.7. The gene discussed is PFKFB4; the disease is breast carcinoma.