We further analyzed gene-gene interactions of deregulated glucose related genes in breast cancer, and it was found that deregulated glucose related genes show high interactions with PFKFB2, PFKFB4, FBP2, PCK2, ADH5 and other genes involved in glucose metabolism (Fig. 8A). The PPI grid was developed by employing STRING and the confidence score value of the network was > 0.7. This evidence concerns the gene PFKFB2 and breast cancer.