At 24 h and 48 h, untransduced T cells failed to eradicate tumor cells, while the transduced OVA-TCR expressing T-cells showed significant cytotoxicity against B16-OVA cells (Fig. 4d) and secreted elevated levels of effector cytokines such as IFN-γ and TNF-α (Fig. 4e). Here, TNF is linked to neoplasm.