HLF and Familial prostate cancer: Although DNA methylation in the gene body has sometimes been found to be associated with increased transcription [16] and two of the top five hypermethylated DMCs were both located in the gene body of HLF, increased methylation in both of the DMCs in HLF was associated with decreased expression of HLF. This inverse association might suggest a potential alternative transcriptional regulation of HLF in prostate cancer instead.