In contrast, mice treated with sEV-MDA231 previously blocked with anti-lactadherin antibody or treated with anti-lactadherin antibody alone developed the same numbers but relatively smaller tumor nodules than non-treated mice (Fig. 8D, E), suggesting that anti-lactadherin treatment and blockade of lactadherin in sEVs play and important role in tumor and ascites formation, in vivo. This evidence concerns the gene MFGE8 and neoplasm.