JAG1 and breast cancer: The cell lines were cultured and subjected to different levels of Notch activation: Elevated Notch signalling (“Notch on”) was accomplished by culturing cells on immobilized Jagged1 ligand, as Jagged1 has been implicated in basal-like breast cancer [17, 19, 20]; blockage of Notch signalling (“Notch off”) was achieved by culturing the cells in the presence of the γ-secretase inhibitor DAPT and DMSO was used for the “ground state” of Notch signalling (see Fig. 1B for a schematic presentation of the assay).