However, our study to some extent indicates significant differences in blood amino acid levels between NHB and non-NHB, and the detection of maternal serum TBA and ALT is expected to be a method for monitoring NHB-related amino acid metabolic disorders, contributing to the early detection of fetal anomalies, the reduction of complications and other adverse pregnancy outcomes, and improvement of prognosis for the affected neonatal. Here, GPT is linked to amino acid metabolism disease.