CBS and posterior cortical atrophy: However, HSF1 and CBS knockdown or inhibition increased diphosphoglycerate, 3-phosphoglycerate, and phosphoenolpyruvate precursors to pyruvate levels, and nearly all glycolysis and TCA cycle metabolites analyzed were increased (Supplementary Fig. 3a), indicating that overall carbon utilization was greatly decreased from CBS and HSF1 and CBS knockdown in PCa.