ITGB4 and neoplasm: Next, we used single simple gene set enrichment analysis (ssGSEA) to assess immune cell infiltration and observed that ITGB4 expression level negatively correlated with tumor purity (r = -0.600, p =  < 0.001, Fig. 6A), and positively correlated with stromal score (r = 0.570, p =  < 0.001, Fig. 6B), ESTIMATE score (r = 0.600, P < 0.001, Fig. 6C) and immune score (r = 0.600, P < 0.001, Fig. 6D), indicating that ITGB4 expression is closely relevant to the tumor microenvironment (TME).