By performing HE, Masson’s trichrome, and Sirius Red staining, we found a significant liver fibrosis and collagen formation in CCl4 mice than in oil mice, while NLRP3−/− mice after CCl4 intervention had significantly weaker fibrosis than the mice in the CCl4 group (Fig. 3a). This evidence concerns the gene NLRP3 and Hepatic fibrosis.