Subsequently, when we performed PCR validation on surgical specimens after neoadjuvant immunotherapy, we found that APOC1 and CEP55 were significantly upregulated and hsa-miR-378a-5p and hsa-miR-378a-3p were considerably downregulated in esophageal squamous cell carcinoma samples. This evidence concerns the gene CEP55 and esophageal squamous cell carcinoma.