In a previous study, we showed that local IL-22 expression in the colon restored colonic sensitivity and normal animal behavior in the C. rodentium infection model of IBS.11 Thus, we investigated the impact of pasteurized A. muciniphila on colonic IL-22 expression using a very sensitive approach, the digital droplet PCR (ddPCR), to increase the detection sensitivity for colonic IL-22 expression. Here, IL22 is linked to irritable bowel syndrome.