Notably, our analysis identified a significant correlation between the prognosis of osteosarcoma patients and four genes: V-set and Immunoglobulin Domain-containing Protein 4 (VSIG4), Membrane Palmitoylated Protein 1 (MPP1), Pleckstrin Homology-domain-containing family O member 2 (PLEKHO2), and Guanylate Binding Protein 2 (GBP2). This evidence concerns the gene PLEKHO2 and osteosarcoma.