ZNF471 and renal carcinoma: To verify the biological function of ZNF471 in renal cancer, we transfected the pcDNA3.1-ZNF471 expressing plasmid into renal cancer cell lines (786-O, ACHN, and caki-1), and then, the ZNF471 overexpression efficiency was analysed by qRT-PCR after transfection for 24 h, 48 h and 72 h.