Highresolution bisulfite genomic sequencing (BGS) analysis was conducted to examine the methylation status of 43 individual CpG sites within the ZNF471 promoter, with a higher density of methylated alleles were observed in RCC cell lines (786-O and ACHN) and RCC tissues compared with normal cell lines (HK-2) and adjacent normal tissues, consistent with the MSP results (Fig. S2I-J). Here, ZNF471 is linked to renal cell carcinoma.