ZNF471 and renal carcinoma: To further verify whether the low expression of ZNF471 in renal cancer tissues is related to the high promoter CpG methylation, we treated renal cancer cell lines with 5-Aza, DNA methyltransferase inhibitor, and the results showed that in renal cancer cell lines, the methylation level of ZNF471 was decreased, while the expression level of ZNF471 was increased (Fig. 1K).