ECHS1 and metastatic malignant neoplasm in the lymph nodes: Additionally, deeply invaded GC tumor tissues (T3-4), lymph node metastasis (LNM), and advanced tumor-node-metastasis (TNM) stage (III-IV) indicated enhanced ECHS1 levels than in T1-2 (P < 0.01, Figure 2B), without LNM (P < 0.05, Figure 2C), and with TNM stage I-II (P < 0.001, Figure 2D), respectively.