Previous studies have shown that irisin inhibited oxidative stress and activation of NLRP3 inflammasomes in Ang II-treated VSMCs by activating AMPK-SIRT1 signaling pathway38 as well as decreased blood pressure levels by alleviating endothelial dysfunction in mesenteric arteries of hypertensive rats via activating the AMPK-Akt-eNOS-NO signaling pathway20. Here, AKT1 is linked to endothelial dysfunction.