To further explore whether CRNDE-induced HCC cells reshaped the TME in vivo, scRNA-seq (10X Genomics) was performed on the subcutaneous tumors of the above Crnde overexpression groups and the control groups, including a total of 17032 single cells (9,112 from the control groups and 7,920 from the Crnde groups) in the final datasets after quality control. Here, CRNDE is linked to hepatocellular carcinoma.