To investigate whether [18F]FSPG could be used as a non-invasive marker of NRF2 expression in vivo, H460 FLuc (NRF2-high) and H1299 FLuc (NRF2-low) NSCLC cell lines were orthotopically grown in the lungs of mice. The gene discussed is NFE2L2; the disease is non-small cell lung carcinoma.