Western Blot as well as qRT-PCR experiments further confirmed the expression of DUX4 and known DUX4-target genes TRIM48, TRIM49, ZSCAN4, ZSCAN5a, ZSCAN5d and RFPL4A upon HSV-1, HCMV and KSHV-infection (Fig. 1C–E, Fig. S2A-G) 25 in different experimental settings. This evidence concerns the gene ZSCAN5A and infection.