We could show that wildtype (wt) HSV-1 infection as well as infection with HSV-1-Δ γ34.5 resulted in DUX4 expression, whereas DUX4 expression is abrogated in cells infected with HSV-1 lacking either ICP0 or ICP4 (Fig. 2B and Fig. S5A). This evidence concerns the gene DUX4 and infection.