To explain possible involvement of pre-existing barcodes in replicate A in dabrafenib insensitivity, we suggest that CNV gain in SGK1 gene found in our study might govern the insensitivity associated with pre-existing barcodes as SGK1 was previously reported as downstream of YAP1 transcriptional activator activating ERK1/2 signalling as a possible mechanism of BRAF V600E insensitivity in CRC cells37. This evidence concerns the gene YAP1 and colorectal carcinoma.