As LSCs mainly reside in CD34+CD38- and CD34+CD38+ compartments3,21, we further examined those subsets of our AML specimens and found that TMIGD2 was more highly expressed on CD34+CD38- and CD34+CD38+ compartments in comparison with CD45dimSSClow unfractionated blasts (Fig. 1c). This evidence concerns the gene CD34 and acute myeloid leukemia.