Transcription factor RFX1 is potentially involved in the triggering of TMIGD2 overexpression in LSCs: first, RFX1 can bind the open chromatin region of TMIGD244; second, TMIGD2 expression is positively correlated with RFX1 expression at mRNA level20; third, RFX1 has been shown to be enriched in FLT3-ITD-specific subclones derived from AML patients, which are able to expand in vitro and engraft in vivo with higher proliferative capacity than the FLT3-WT subclones11. Here, FLT3 is linked to acute myeloid leukemia.