In agreement with cBioPortal database (Supplementary Fig. 6e)31, cDNA sequencing of cell line and primary samples showed limited alterations within intracellular domain (IC) and transmembrane domain (TM) of TMIGD2 (Supplementary Fig. 6f), suggesting that a gain-of-function mutation is unlikely the cause of TMIGD2 signaling in AML. Here, TMIGD2 is linked to acute myeloid leukemia.