In addition, the dominant cellular phenotypes that resulted from TMIGD2 knockdown included a substantial induction of apoptosis (Fig. 2d and Supplementary Fig. 2f), a prominent G0/G1 cell-cycle arrest (Fig. 2e, Supplementary Fig. 2g, h), a noticeable increase of differentiation (Fig. 2f, g and Supplementary Fig. 2i–k) and a significant decrease of colony-forming cell (CFC) number (Fig. 2h, Supplementary Fig. 2l) in Kasumi-1, HEL and K562 AML cells. This evidence concerns the gene TMIGD2 and acute myeloid leukemia.