Importantly, GSEA showed that the top seven pathways enriched in CD34+TMIGD2+ fraction consisted of E2F targets, MYC targets, and G2M checkpoints (Fig. 4f, g), which was consistent with our findings that CD34+TMIGD2+ cells generated more colonies and induced leukemia much more efficiently than TMIGD2− counterparts (Fig. 4b–d). This evidence concerns the gene TMIGD2 and leukemia.