To confirm whether PDPN could affect migration and invasion of melanoma cells in wnt/β-catenin signaling pathway-dependent manner, A375 cells with PDPN knockdown were treated with a Wnt/β-catenin signaling pathway agonist (SKL2001) and PDPN overexpression A875 cells were treated with an inhibitor (IWR1) [38]. This evidence concerns the gene PDPN and melanoma.