As expected, TC2N could inhibit FASN protein expression in these cell lines (Additional file 9: Fig. S3E), suggesting the effect of TC2N on FASN expression may be independent of BC molecular subtype, and the expression of TC2N did not show statistical correlation with FASN expression in all of the subtypes of BC probably because the sample size of each molecular subtype of BC is too small to analyze. This evidence concerns the gene FASN and breast cancer.