FASN and breast cancer: Next, to confirm the key domain of TC2N is responsible for its interaction with FASN, co-IP assays were performed in BC cells infected with TC2N full-length, TC2N-C2A del (lack C2A domain of TC2N, C2A-del), TC2N-C2B del (lack C2B domain of TC2N, C2B-del) and TC2N-C2A + C2B del (lack C2A and C2B domain of TC2N, C2A + C2B-del) lentivirus, respectively (Fig. 5G).