To investigate the specific impact of IGF2BP3 on neutrophil function, we established three in vitro co-culture models of glioma cells with neutrophils from distinct sources: tumor-associated neutrophils (TANs) from glioma tissue in mice with in situ tumorigenesis (Fig. 1a), bone marrow neutrophils from healthy mice (labeled as “neutrophil”; Supplementary Fig. 1c), and human HL-60 cells induced to exhibit neutrophil-like characteristics (called “induced neutrophils”; Supplementary Fig. 1d). This evidence concerns the gene IGF2BP3 and glioma.