In addition, multiplex immunofluorescence staining or multiplexed ion beam imaging (MIBI) (Supplementary Fig. 5b, c) showed that R270H mutp53-expressing 4T1 tumors had less infiltration of CD3+CD8+ and CD3+ T cells, granzyme B+ cells, dendritic cells (DCs) and lymphoid tissue-resident CD11b+ classical dendritic cells (cDCs) in BALB/c mice compared with the control (Fig. 5e–m), suggesting that expression of R270H mutp53 engendered a immunosuppressive tumor microenvironment (TME) in 4T1 tumors. This evidence concerns the gene CD8A and neoplasm.