Interestingly, OTUB2-IN-1 treatment only significantly reduced the expression of phosphorylated Akt in B16-F10 tumors (Supplementary Fig. 27a, b) and significantly reduced the expression of phosphorylated p65 in KLN205 tumors (Supplementary Fig. 28a, b), suggesting that OTUB2 may possess more general roles in regulation of PD-L1 in various cancer types. Here, AKT1 is linked to cancer.