We mixed Kasumi-1 (an AML line harboring AML1::ETO and homozygous TP53R248G) with K562 (a chronic myeloid leukemia [CML] line harboring BCR::ABL1 and monoallelic TP53Q136fs) cells at four defined ratios (100:1, 15:1, 1.5:1, 1:100) (Fig. 2d); at each ratio, we amplified and sequenced the homozygous TP53R248G mutation found in Kasumi-1 cells21. The gene discussed is RUNX1; the disease is acute myeloid leukemia.